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Cell Preparation for Single Cell Protocols - 10x Genomics
https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/sample-prep/single-cell-protocols-cell-preparation-guide
WebJun 23, 2023 · CG000053_CellPrepGuide_RevD.pdf. View and download. 10x Genomics Single Cell protocols require a suspension of viable single cells or nuclei as input. Minimizing the presence of cellular aggregates, dead cells, noncellular nucleic acids, and potential biochemical inhibitors of reverse transcription is critical to obtaining high-quality data.
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Cell Preparation for Single Cell Protocols
https://cdn.10xgenomics.com/image/upload/v1686678481/support-documents/CG00053_Handbook_CellPreparation_SingleCellProtocols_Rev_D.pdf
Web10x Genomics Single Cell protocols require a suspension of viable single cells or nuclei as input. Minimizing the presence of cellular aggregates, dead cells, noncellular nucleic acids, and potential biochemical inhibitors of reverse transcription is …
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USER GUIDE Chromium Next GEM Single Cell 3ʹ Reagent …
https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf
WebThe Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling 500-10,000 individual cells per sample. A pool of ~3,500,000 10x Barcodes are sampled separately to index each cell’s transcriptome.
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Chromium Single Cell 5' Reagent Kits User Guide (v2 ... - 10x Genomics
https://www.10xgenomics.com/support/single-cell-immune-profiling/documentation/steps/library-prep/chromium-single-cell-5-reagent-kits-user-guide-v-2-chemistry-dual-index
WebSep 9, 2022 · 10x Genomics Support / Single Cell Immune Profiling / Documentation / Library Construction / Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) User Guide, CG000331. CG000331_ChromiumNextGEMSingleCell5-v2_UserGuide_RevE.pdf. View and download.
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Chromium Single Cell 3' Reagent Kits User Guide (v3.1 ... - 10x Genomics
https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/library-prep/chromium-single-cell-3-reagent-kits-user-guide-v-3-1-chemistry-dual-index
WebNov 7, 2022 · View and download. FOR USE WITH: Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns PN-1000268. Chromium Next GEM Single Cell 3ʹ Kit v3.1, 4 rxns PN-1000269. Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120. Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127.
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USER GUIDE Chromium Next GEM Automated Single Cell 3 …
https://cdn.10xgenomics.com/image/upload/v1686293300/support-documents/CG000286_ChromiumSingleCell3__v3.1_Automation_UG_RevF.pdf
WebDocument NumberCG000286. Title. Chromium Next GEM Automated Single Cell 3' Reagent Kits v3.1 User Guide. RevisionRev E to Rev F. Revision DateMay 2023. Specific Changes: • Updated to include information regarding two different sample input volumes (pages 19, 21, 26-27, 30, 33-35).
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Getting Started: Single Cell 3’ Gene Expression - 10x Genomics
https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000360_GettingStartedSingleCell3-GeneExpression_RevA.pdf
WebThe Chromium Single Cell Gene Expression Solution provides a scalable microfluidic platform for gene expression profiling of 500-10,000 individual cells per sample. A pool of ~3,500,000 10x Barcodes are sampled separately to index each cell’s transcriptome.
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Library - 10x Genomics
https://www.10xgenomics.com/library
WebThe Library houses 10x Genomics Product Literature, Posters, Application Notes, and Research Snapshots. These documents span across all areas of interest, product, instrument, and application lines, available in English, Chinese, and Japanese.
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10x Genomics® Sample Preparation Demonstrated Protocol
https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG00054_SamplePrepDemonstratedProtocol_-_CultCells_RevB.pdf
Web10x Genomics®. Sample Preparation Demonstrated Protocol. Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing. NOTICES. Notices. Manual Part Number. CG00054. Rev B. Legal Notices. 2017 10x Genomics, Inc. All rights reserved.
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10x Genomics® Single Cell Protocols - Gene Expression Center
https://gec.biotech.wisc.edu/wp-content/uploads/sites/1676/2021/07/cg00053_cellprepguide_revc.pdf
WebThis Cell Preparation Guide describes best practices and general protocols for washing, counting and concentrating cells from both abundant and limited cell suspensions (greater than or less than 100000 total cells, respectively) in preparation for use in 10x Genomics Single Cell Protocols.
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